OPTIMIZATION OF CULTURE CONDITIONS FOR KOJIC ACID PRODUCTION IN SURFACE FERMENTATION BY ASPERGILLUS ORYZAE ISOLATED FROM WHEAT GRAINS

Kojic acid is a natural organic synthesized during aerobic fermentation of carbohydrates as secondary metabolite by some species Aspergillus and has been used commercially in several industrial applications. The current investigation aims to optimize the culture conditions for kojic production from starch carbon source novel isolate oryzae 1034. Seventy-five isolates representing 11 were isolated stored wheat grains screened biosynthesis. Amongst, A. 1034 was selected most potent producer starch. This strain subjected different maximize versus glucose. results concluded that glucose substrates concentrations 60 80 g/l, respectively optima production. optimum phosphorus concentration 0.5 2.0 g/l KH2PO4 media, respectively. maximum yield attained at 28oC days incubation both media with pH 4.5 5.0, Supplementation Pb+2 medium Zn+2 stimulated biosynthesis 79.3 68.8 Alternatively, decreased supplementation amino acids medium. These findings suggest possibility using promising.